SLAS

A microfluidic dispenser

From LabAutopedia

A Chip-to-chip Nanoliter Microfluidic Dispenser

Jianbin Wang, Ying Zhou, Haiwei Qiu, Huang Huang, Changhong Sun, Jianzhong Xi and Yanyi Huang
College of Engineering, Peking University, Beijing 100871, China


A high-throughput microfluidic device is developed to handle liquid dispensation in nanoliter range. The dispenser system shows no cross-contamination between the microreserviors, indicating its great potential in large-scale screening experiments. Using the dispenser, cells are parallelly dispensed into microdams, cultured for several hours, and stained with Calcein AM to determine their viability, indicating its great potential in cell related operations. An array of 115 nl PCR reactions, as well as the single channel addressable chip further demonstrate the high flexibility and wide applications of this novel system. It provides an inexpensive and compact solution for liquid sample distribution in the range of tens to hundreds of nanoliters.