MP23:Vogel:A Microfluidic Based Vacular Mimic
From LabAutopedia
Paul A. Vogel
Michigan State University
A Microfluidic Based Vascular Mimic Capable of Transendothelial Electronic Resistance Measurements and Post-Growth Experimentation
Transendothelial electronic resistance (TEER) measurements are performed across a cell layer immobilized in a microfluidic device in a manner that also enables the cell layer to interact with a flowing stream of red blood cells (RBCs). A bipolar pulsed square wave potential is applied across a monolayer of bovine pulmonary artery endothelial cells, and the resulting current response is measured and integrated to yield TEER signal. After cell seeding on the device, a decrease in TEER signal from 22.3 ± 1.6 μC to 3.5 ± 0.4 μC (corresponding to a resistance of 40.9 ± 2.9 Ω•cm2 to 259.1 ± 27.4 Ω•cm2) was observed after 8 h of cell growth, showcasing the ability to monitor cell barrier integrity. Subsequent to determination of cell confluence, a variety of postgrowth experiments can be performed, including, but not limited to, drug transport across the cell monolayer. For example, C-peptide, a 31 amino acid peptide that is co-secreted with insulin, has been shown to reduce neuropathies in people with type 1 diabetes. However, it is unknown if the peptide exerts its effects directly by crossing the blood brain barrier, or if these effects are a result of C-peptide activity in the bloodstream. Here, results will be presented providing new information about C-peptide’s mechanism of action. Moreover, the data will demonstrate that this device may also serve as novel tool for microfludic pharmacological distribution studies.
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